6GBQ
Crystal Structure of the oligomerization domain of Vp35 from Reston virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.27982 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.230, 45.583, 110.777 |
| Unit cell angles | 90.00, 101.66, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.430 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gbr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS (Nov 1, 2016) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | MOLREP (11.4.06) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.520 | 47.520 | 2.520 |
| High resolution limit [Å] | 2.430 | 9.090 | 2.430 |
| Rmerge | 0.059 | 0.048 | 0.917 |
| Rmeas | 0.064 | 0.053 | 0.996 |
| Rpim | 0.025 | 0.021 | 0.384 |
| Total number of observations | 172008 | ||
| Number of reflections | 26031 | 514 | 2731 |
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 98.9 | 94.9 | 99.4 |
| Redundancy | 6.6 | 5.6 | 6.6 |
| CC(1/2) | 0.999 | 0.998 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 20 % PEG-6000: 100 mM Na-acetate |
