6GBO
Crystal Structure of the oligomerization domain of Vp35 from Ebola virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0080 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.782, 98.907, 185.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.2123 |
| Rwork | 0.211 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gbp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS (VERSION Oct 15, 2015) |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.450 | 49.450 | 2.150 |
| High resolution limit [Å] | 2.100 | 10.060 | 2.100 |
| Rmerge | 0.107 | 0.032 | 1.633 |
| Rmeas | 0.112 | 0.034 | 1.703 |
| Rpim | 0.031 | 0.010 | 0.477 |
| Total number of observations | 902583 | ||
| Number of reflections | 69399 | 735 | 4358 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 99.9 | 98.9 | 98.9 |
| Redundancy | 13 | 10.9 | 12.4 |
| CC(1/2) | 0.999 | 0.999 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 296 | 50 mM HEPES-NaOH pH 7.5, 2.5 M Na-acetate |
