6GB9
The Structure of variant S328A of the Mo-insertase domain Cnx1E from Arabidopsis thaliana in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.020, 123.800, 132.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.407 - 1.670 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g2r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | STARANISO (1.10.9) |
Phasing software | PHASER (2.8.1) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.407 | 1.841 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.119 | 0.772 |
Rmeas | 0.154 | 0.991 |
Rpim | 0.097 | 0.615 |
Number of reflections | 41927 | 2096 |
<I/σ(I)> | 7 | 1.7 |
Completeness [%] | 66.1 | 13.2 |
Redundancy | 4.5 | 4.5 |
CC(1/2) | 0.990 | 0.675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M sodium formate, 0.1 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate, 0.1 M sodium potassium tartrate tetrahydrate, 0.1 M sodium oxamate; 0.1 M Tris BICINE pH 8.5; 10 % (w/v) PEG 4000, 20 % (v/v) glycerol |