6GB6
Structure of H-2Kb with dipeptide GL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.630, 82.240, 89.550 |
| Unit cell angles | 90.00, 111.29, 90.00 |
Refinement procedure
| Resolution | 83.440 - 1.780 |
| R-factor | 0.20828 |
| Rwork | 0.207 |
| R-free | 0.23227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.346 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.440 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.066 | 0.488 |
| Number of reflections | 82002 | 6083 |
| <I/σ(I)> | 12.3 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 2.15 M Na/K phosphate, 15% MPD |
