6GB0
The Structure of variant K294A of the Mo-insertase domain Cnx1E from Arabidopsis thaliana in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.470, 123.420, 132.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.060 - 1.814 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g2r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | STARANISO (1.10.9) |
Phasing software | PHASER (2.8.1) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.791 | 2.089 |
High resolution limit [Å] | 1.814 | 1.814 |
Rmerge | 0.089 | 0.665 |
Rmeas | 0.115 | 0.854 |
Rpim | 0.072 | 0.532 |
Number of reflections | 32849 | 2737 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 66.3 | 16.2 |
Redundancy | 4.4 | 4.7 |
CC(1/2) | 0.995 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M imidazole, 0.1 M MES pH 6.5; 0.06 M sodium nitrate, 0.06 M sodium phosphate, 0.06 M ammonium sulfate; 20 % (v/v) PEG 500 MME, 10 % PEG 8000 |