6GAY
A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49I mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.675, 56.675, 156.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.889 - 1.860 |
R-factor | 0.1679 |
Rwork | 0.166 |
R-free | 0.20920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kuk |
RMSD bond length | 0.020 |
RMSD bond angle | 1.624 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1-2155_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.890 | 1.900 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.061 | |
Rmeas | 0.065 | |
Number of reflections | 22330 | 1358 |
<I/σ(I)> | 23.2 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.2 | 9.2 |
CC(1/2) | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292.15 | 0.1 M Na-acetate, 0.1 M Ammonium sulfate, 0.3 M Sodium formate, 3 % PGA-LM, 5 % (w/v) PEG 8000 |