6G8Y
Crystal Structures of the Single PDZ Domains from GRASP65 and their Interaction with the Golgin GM130
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 53.443, 53.443, 50.923 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.720 - 1.400 |
R-factor | 0.16519 |
Rwork | 0.163 |
R-free | 0.21006 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.452 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmeas | 0.042 | 0.740 |
Number of reflections | 16914 | 1228 |
<I/σ(I)> | 22.96 | 2.82 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.0 M NaCl, 100 mM Bis-Tris, pH 5.5 |