6G8W
Crystal Structures of the Single PDZ Domains from GRASP65 and their Interaction with the Golgin GM130
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.825, 82.613, 49.220 |
| Unit cell angles | 90.00, 110.82, 90.00 |
Refinement procedure
| Resolution | 26.850 - 2.120 |
| R-factor | 0.18839 |
| Rwork | 0.186 |
| R-free | 0.23548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.850 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmeas | 0.078 | 0.626 |
| Number of reflections | 10762 | 788 |
| <I/σ(I)> | 15.78 | 2.97 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.4M Na/K phosphate pH 8.2 |
