6G8E
Crystal Structure of the Amyloid-like VTQVGF segment from the R5 repeat of the E. coli Biofilm-associated CsgA Curli protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8729 |
Spacegroup name | P 1 |
Unit cell lengths | 4.810, 19.340, 21.900 |
Unit cell angles | 65.82, 83.72, 83.20 |
Refinement procedure
Resolution | 19.930 - 1.700 |
R-factor | 0.1235 |
Rwork | 0.123 |
R-free | 0.13200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ideal beta-strand |
RMSD bond length | 0.017 |
RMSD bond angle | 1.529 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.930 | 19.930 | 1.820 |
High resolution limit [Å] | 1.700 | 4.810 | 1.700 |
Rmerge | 0.275 | 0.211 | 0.442 |
Rmeas | 0.298 | 0.228 | 0.481 |
Total number of observations | 5372 | ||
Number of reflections | 776 | 25 | 136 |
<I/σ(I)> | 5.02 | 6.73 | 3.18 |
Completeness [%] | 98.4 | 100 | 99.3 |
Redundancy | 6.923 | 6.92 | 6.522 |
CC(1/2) | 0.979 | 0.987 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Reservoir contained 3.0 M Sodium chloride and 0.1 M BIS-Tris pH 5.5 |