6G8E

Crystal Structure of the Amyloid-like VTQVGF segment from the R5 repeat of the E. coli Biofilm-associated CsgA Curli protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8729
Spacegroup name	P 1
Unit cell lengths	4.810, 19.340, 21.900
Unit cell angles	65.82, 83.72, 83.20

Refinement procedure

Resolution	19.930 - 1.700
R-factor	0.1235
Rwork	0.123
R-free	0.13200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ideal beta-strand
RMSD bond length	0.017
RMSD bond angle	1.529
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.930	19.930	1.820
High resolution limit [Å]	1.700	4.810	1.700
Rmerge	0.275	0.211	0.442
Rmeas	0.298	0.228	0.481
Total number of observations	5372
Number of reflections	776	25	136
<I/σ(I)>	5.02	6.73	3.18
Completeness [%]	98.4	100	99.3
Redundancy	6.923	6.92	6.522
CC(1/2)	0.979	0.987	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Reservoir contained 3.0 M Sodium chloride and 0.1 M BIS-Tris pH 5.5