6G8C
Crystal Structure of the Amyloid-like IYQYGG segment from the R1 repeat of the E. coli Biofilm-associated CsgA Curli protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 42.170, 4.760, 19.600 |
Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
Resolution | 19.140 - 1.650 |
R-factor | 0.1478 |
Rwork | 0.145 |
R-free | 0.17790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ideal beta-strand |
RMSD bond length | 0.019 |
RMSD bond angle | 1.673 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.140 | 19.140 | 1.850 |
High resolution limit [Å] | 1.650 | 3.690 | 1.650 |
Rmerge | 0.099 | 0.054 | 0.326 |
Rmeas | 0.111 | 0.062 | 0.359 |
Number of reflections | 545 | 59 | 141 |
<I/σ(I)> | 8.66 | 16.47 | 3.21 |
Completeness [%] | 97.3 | 90.8 | 100 |
Redundancy | 5.231 | 4.237 | 5.901 |
CC(1/2) | 0.996 | 0.996 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Reservoir contained 0.1 M Sodium acetate pH 4.6 and 2.0 M Sodium formate |