6G8B
E. coli Aminopeptidase N solved by Native SAD from a dataset collected in 60 second with JUNGFRAU detector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-23 |
| Detector | PSI JUNGFRAU 1M |
| Wavelength(s) | 2.0667 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.109, 122.109, 172.021 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.786 - 2.374 |
| R-factor | 0.1617 |
| Rwork | 0.159 |
| R-free | 0.20590 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.520 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.787 | 2.520 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmeas | 0.062 | 0.180 |
| Number of reflections | 108726 | 12294 |
| <I/σ(I)> | 34.06 | 6.07 |
| Completeness [%] | 93.8 | 65.8 |
| Redundancy | 14.2 | 3.4 |
| CC(1/2) | 0.990 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.8M Sodium malonate |
