6G8B
E. coli Aminopeptidase N solved by Native SAD from a dataset collected in 60 second with JUNGFRAU detector
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-23 |
Detector | PSI JUNGFRAU 1M |
Wavelength(s) | 2.0667 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 122.109, 122.109, 172.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.786 - 2.374 |
R-factor | 0.1617 |
Rwork | 0.159 |
R-free | 0.20590 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.520 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.787 | 2.520 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmeas | 0.062 | 0.180 |
Number of reflections | 108726 | 12294 |
<I/σ(I)> | 34.06 | 6.07 |
Completeness [%] | 93.8 | 65.8 |
Redundancy | 14.2 | 3.4 |
CC(1/2) | 0.990 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.8M Sodium malonate |