6G8A
Lysozyme solved by Native SAD from a dataset collected in 5 seconds at 1 A wavelength with JUNGFRAU detector
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-16 |
Detector | PSI JUNGFRAU 1M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.903, 78.903, 36.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.262 - 1.143 |
R-factor | 0.1132 |
Rwork | 0.112 |
R-free | 0.13040 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.380 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.450 | 1.210 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmeas | 0.037 | 0.220 |
Number of reflections | 77341 | 9912 |
<I/σ(I)> | 41.82 | 4.95 |
Completeness [%] | 95.8 | 75.8 |
Redundancy | 15.1 | 3.4 |
CC(1/2) | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% PEG MME 5000, 2 M NaCl, 50 mM Sodium Acetate pH 4.5, 25% 480 ethylene glycol |