6G87
Flavonoid-responsive Regulator FrrA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979031 |
| Spacegroup name | P 43 |
| Unit cell lengths | 119.592, 119.592, 78.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.560 - 2.920 |
| R-factor | 0.18383 |
| Rwork | 0.183 |
| R-free | 0.21112 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.570 | 3.100 |
| High resolution limit [Å] | 2.919 | 2.919 |
| Rmeas | 0.101 | 0.931 |
| Number of reflections | 47028 | 7487 |
| <I/σ(I)> | 15.17 | 1.96 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 7.02 | 7.17 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 6mg/ml FrrA in 20 mM Na2HPO4, 100 mM imidazole, pH 7.5, 50 mM NaCl. Precipitant 20 % PEG 8000; 0.1 M CHES, pH 9.5. |
