6G86

Structure of Cdc14 bound to SIC1 PxL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.28
Spacegroup name	P 21 21 21
Unit cell lengths	75.090, 94.321, 127.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.800 - 1.740
R-factor	0.1671
Rwork	0.166
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohc
RMSD bond length	0.010
RMSD bond angle	1.017
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.800	1.800
High resolution limit [Å]	1.740	1.740
Rmerge	0.060	0.950
Rpim		0.500
Number of reflections	93481	42727
<I/σ(I)>	11.8	1.65
Completeness [%]	99.6	98.5
Redundancy	5.1	4.7
CC(1/2)	0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES pH6, 18% PEG200, 5% PEG 3000