6G84

Structure of Cdc14 bound to CBK1 PxL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.92
Spacegroup name	C 1 2 1
Unit cell lengths	174.500, 73.930, 83.530
Unit cell angles	90.00, 114.27, 90.00

Refinement procedure

Resolution	55.290 - 2.470
R-factor	0.2283
Rwork	0.226
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohc
RMSD bond length	0.002
RMSD bond angle	0.415
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.290	2.550
High resolution limit [Å]	2.470	2.470
Rmerge	0.130	1.470
Rmeas		1.600
Rpim	0.050	0.630
Number of reflections	34914	3456
<I/σ(I)>	8.16	1.473
Completeness [%]	99.7	99.68
Redundancy	6.5	6.3
CC(1/2)	0.996	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	11% PEG3350, 0.2M Calcium Acetate