6G7W

Crystal structure of NHL repeat containing domain of human NHLRC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	52.631, 46.491, 70.302
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	46.331 - 1.746
R-factor	0.1565
Rwork	0.155
R-free	0.18920
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.331	46.331	1.460
High resolution limit [Å]	1.420	6.360	1.420
Rmerge	0.048	0.022	1.025
Rmeas	0.058	0.026	1.439
Number of reflections	42106	721	260
<I/σ(I)>	12.73	40.45	0.45
Completeness [%]	67.5	95.8	5.7
Redundancy	2.917	2.999	1.558
CC(1/2)	0.999	0.999	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M Li2SO4, 0.1 M BisTris pH 5.5, 25% w/v PEG 3350