6G78
RSK4 N-terminal Kinase Domain S232E in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.979480 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.015, 110.614, 66.559 |
Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
Resolution | 28.515 - 2.500 |
R-factor | 0.2265 |
Rwork | 0.226 |
R-free | 0.23960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g77 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.520 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.025 | |
Number of reflections | 26061 | 857 |
<I/σ(I)> | 2.36 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 20 % PEG 6000, 0.1 M TRIS pH8, 2 mM Zinc chloride |