6G70
Structure of murine Prpf39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.490, 72.760, 207.100 |
| Unit cell angles | 90.00, 112.46, 90.00 |
Refinement procedure
| Resolution | 47.848 - 3.300 |
| R-factor | 0.2507 |
| Rwork | 0.247 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.802 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.450 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 0.149 | 2.600 |
| Number of reflections | 39170 | 4809 |
| <I/σ(I)> | 9.4 | 0.9 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 4.7 | 4.8 |
| CC(1/2) | 0.998 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.1 M bis-tris-propane pH 6.5, 1.8 M sodium acetate |
