6G6S
Crystal structure of human Acinus RNA recognition motif domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918410 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.359, 67.911, 80.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.070 - 1.650 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x1f |
RMSD bond length | 0.017 |
RMSD bond angle | 1.780 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.070 | 80.070 | 1.740 |
High resolution limit [Å] | 1.650 | 4.910 | 1.650 |
Rmeas | 0.071 | 0.024 | 0.864 |
Number of reflections | 19003 | 864 | 3228 |
<I/σ(I)> | 16.3 | 46.1 | 1.97 |
Completeness [%] | 91.6 | 97.7 | 98.5 |
Redundancy | 4.76 | ||
CC(1/2) | 0.999 | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.2 M Ammonium acetate, 100 mM Tris-HCl pH 8.5, 25% w/v Polyethylene glycol 3350 |