6G6J

The crystal structures of Human MYC:MAX bHLHZip complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	74.319, 145.180, 48.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.554 - 2.250
R-factor	0.2069
Rwork	0.205
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knp
RMSD bond length	0.006
RMSD bond angle	0.677
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.930	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.098	0.545
Rmeas	0.109	0.608
Rpim	0.048	0.265
Number of reflections	25627	3679
<I/σ(I)>	8.9	2.8
Completeness [%]	99.7	99.7
Redundancy	4.8	4.9
CC(1/2)	0.997	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	20% (w/v) PEG 3350 0.2M sodium sulfate decahydrate, pH 7