6G67
Crystal structure of a parallel eight-helix coiled coil CC-Type2-II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-10-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 48.110, 48.110, 126.969 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.988 - 1.770 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.968 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.480 | 63.480 | 1.810 |
High resolution limit [Å] | 1.770 | 9.030 | 1.770 |
Rmerge | 0.089 | 0.051 | 0.832 |
Rmeas | 0.092 | 0.054 | 0.855 |
Rpim | 0.021 | 0.016 | 0.193 |
Number of reflections | 7676 | 82 | 414 |
<I/σ(I)> | 17.9 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 20.1 | 14.6 | 19.2 |
CC(1/2) | 0.998 | 0.996 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Sodium citrate tribasic dihydrate, 50 mM Sodium cacodylate and 15% v/v 2-Propanol |