6G64

MamM CTD H264A-E289A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-06-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	37.112, 95.086, 53.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.540 - 1.900
R-factor	0.2084
Rwork	0.205
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.040	0.033	0.240
Rmeas	0.042	0.035	0.273
Rpim	0.014	0.012	0.125
Number of reflections	7559	432	338
<I/σ(I)>	16.2
Completeness [%]	97.2	97.7	88.9
Redundancy	8.5	8.7	4
CC(1/2)		0.999	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH=5.5, 22% PEG 3350, 12.7 uM MnCl2