6G61
Crystal structure of thioredoxin O1 from Arabidopsis thaliana in oxidized state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9799 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.151, 39.242, 79.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.670 - 1.800 |
R-factor | 0.2207 |
Rwork | 0.220 |
R-free | 0.23960 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.474 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((dev_2722: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.670 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.122 | 0.242 |
Rmeas | 0.131 | 0.283 |
Rpim | 0.049 | 0.145 |
Number of reflections | 11316 | 653 |
<I/σ(I)> | 13.1 | 7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.4 | 7 |
CC(1/2) | 0.996 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277 | 100 mM HEPES buffer (pH 7.5) 20% PEG 8000 |