6G5C
Interaction of TiO2 nanoparticles (NM-101) with Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Agilent SuperNova |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2018-03-01 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.8 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.866, 78.866, 36.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.500 - 1.800 |
| R-factor | 0.16733 |
| Rwork | 0.166 |
| R-free | 0.20418 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.855 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.500 | 4.800 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | |
| Number of reflections | 10691 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 20 mg/ml Lys in TiO2 2.56 mg/ml (JRC NM-101 ) Well/solution 10% (w/v) sodium chloride 0.1 M Sodium acetate buffer pH 5.0 25% (v/v) ethylene glycol drop 4 microliter Lys + 2 microliter TiO2 |
