6G55
MamM CTD C267S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97631 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.305, 94.371, 53.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.430 - 1.650 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.21510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5x |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.430 | 35.430 | 1.680 |
High resolution limit [Å] | 1.650 | 9.030 | 1.650 |
Rmerge | 0.039 | 0.024 | 1.450 |
Rmeas | 0.044 | 0.029 | 1.635 |
Rpim | 0.020 | 0.015 | 0.737 |
Number of reflections | 11722 | 85 | 575 |
<I/σ(I)> | 16.8 | ||
Completeness [%] | 99.5 | 97.3 | 97.9 |
Redundancy | 4.6 | 3.9 | 4.5 |
CC(1/2) | 0.999 | 1.000 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M LiSu, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350 |