6G49
Crystal structure of the periplasmic domain of TgpA from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-01 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.640, 71.640, 164.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.500 - 1.600 |
R-factor | 0.1621 |
Rwork | 0.160 |
R-free | 0.19391 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.814 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.500 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.064 | 1.468 |
Rpim | 0.032 | 0.682 |
Number of reflections | 57370 | 4156 |
<I/σ(I)> | 17.6 | 1.66 |
Completeness [%] | 99.9 | |
Redundancy | 7.5 | |
CC(1/2) | 0.999 | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 293 | 0.1 M HEPES sodium pH 7.5 0.8 M Sodium phosphate monobasic monohydrate 0.8 M Potassium phosphate monobasic |