6G43
Crystal structure of SeMet-labeled mavirus major capsid protein lacking the C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 135.350, 135.300, 136.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 2.400 |
R-factor | 0.2145 |
Rwork | 0.213 |
R-free | 0.23360 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.104 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.000 | 48.000 | 2.500 |
High resolution limit [Å] | 2.400 | 20.000 | 2.400 |
Rmerge | 0.118 | 0.041 | 0.609 |
Rmeas | 0.122 | 0.043 | 0.633 |
Number of reflections | 188659 | 309 | 21723 |
<I/σ(I)> | 20.39 | 56.58 | 5.51 |
Completeness [%] | 100.0 | 93.4 | 100 |
Redundancy | 13.816 | 14.269 | 13.323 |
CC(1/2) | 0.999 | 0.999 | 0.931 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1 M tri-sodium citrate, 1.2 - 1.7 M ammonium sulfate |