6G3W

Crystal structure of the BIR3 - SERK2 complex from Arabidopsis thaliana.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000027
Spacegroup name	P 21 21 21
Unit cell lengths	50.184, 52.155, 308.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.410 - 2.200
R-factor	0.21852
Rwork	0.217
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fg8 4z61
RMSD bond length	0.010
RMSD bond angle	1.434
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	2.330
High resolution limit [Å]	2.200	2.200
Rmeas	0.115	1.670
Number of reflections	42439	6697
<I/σ(I)>	17	1.4
Completeness [%]	99.9	99.7
Redundancy	13	13
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	25% (w/v) PEG 3,350, 0.2 M MgCl_2 x 6H_2O, 0.1 M Bis-Tris pH 6.5