6G3U
Structure of Pseudomonas aeruginosa Isocitrate Dehydrogenase, IDH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.460, 149.020, 201.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.618 - 2.707 |
| R-factor | 0.2106 |
| Rwork | 0.208 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zda |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.176 | 0.891 |
| Number of reflections | 51799 | 3682 |
| <I/σ(I)> | 19 | 3.7 |
| Completeness [%] | 98.0 | 97.2 |
| Redundancy | 18.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | PEG 3350, Sodium phosphate monobasic, glycerol, NADP |
