6G3T
X-ray structure of NSD3-PWWP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.178, 88.091, 79.988 |
| Unit cell angles | 90.00, 89.88, 90.00 |
Refinement procedure
| Resolution | 29.610 - 2.530 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6g3p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 2.571 |
| High resolution limit [Å] | 2.528 | 2.528 |
| Rmerge | 0.135 | 0.898 |
| Rmeas | 0.147 | 0.976 |
| Rpim | 0.056 | 0.380 |
| Number of reflections | 21054 | 3076 |
| <I/σ(I)> | 13.2 | 2.342 |
| Completeness [%] | 100.0 | 69.5 |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 0.996 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM HEPES pH 7.8, 28% PEG3350, 2% PEG200, 1.5% 1,2-Butandiol |
