6G2P

Crystal structure of L-tryptophan oxidase VioA from Chromobacterium violaceum in complex with L-tryptophan

Replaces:  6ESE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.928
Spacegroup name	C 2 2 21
Unit cell lengths	152.092, 175.338, 94.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.046 - 2.600
R-factor	0.1801
Rwork	0.178
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6esd
RMSD bond length	0.009
RMSD bond angle	1.021
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.046	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.047
Rmeas	0.053
Number of reflections	38572
<I/σ(I)>	10.09
Completeness [%]	98.0
Redundancy	4.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES pH 7.5, 9% PEG 8000 and 9% ethylene glycol