6G2G
Fe-S assembly Cfd1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.780, 90.434, 125.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.880 - 2.570 |
| Rwork | 0.232 |
| R-free | 0.26840 |
| Structure solution method | SAD |
| RMSD bond length | 0.044 |
| RMSD bond angle | 1.945 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.880 | 2.660 |
| High resolution limit [Å] | 2.566 | 2.570 |
| Rmerge | 0.052 | 0.893 |
| Rmeas | 0.061 | |
| Rpim | 0.529 | |
| Number of reflections | 21847 | 2093 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.4 | 96.59 |
| Redundancy | 3.6 | 3.8 |
| CC(1/2) | 0.999 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Na-HEPES (pH 7.0), 1.5 M ammonium sulfate |
