6G28
Human [protein ADP-ribosylargenine] hydrolase ARH1 in complex with ADP-ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9760 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.102, 66.687, 83.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.090 - 1.230 |
R-factor | 0.14566 |
Rwork | 0.145 |
R-free | 0.15744 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hfw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.527 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.690 | 1.250 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.059 | 0.262 |
Rmeas | 0.064 | 0.295 |
Rpim | 0.025 | 0.132 |
Number of reflections | 103868 | 4932 |
<I/σ(I)> | 21.7 | 5.5 |
Completeness [%] | 99.6 | 97.1 |
Redundancy | 12.2 | 9.3 |
CC(1/2) | 0.998 | 0.981 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM magnesium formate, 20 % (w/v) PEG3350 |