6G24
X-ray structure of NSD3-PWWP1 in complex with compound 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.541800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.213, 46.741, 63.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.950 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.950 | 2.139 |
| High resolution limit [Å] | 2.103 | 2.103 |
| Rmerge | 0.057 | 0.570 |
| Number of reflections | 7983 | |
| <I/σ(I)> | 22.4 | 2.5 |
| Completeness [%] | 96.5 | 74 |
| Redundancy | 6.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100mM Morpheus Buffer 3, 30% P550MME_P20K, 10% Morpheus Ethylene glycols |
