6G1T
TraN, a repressor of an Enterococcus conjugative type IV secretion system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.770, 44.170, 56.460 |
Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
Resolution | 41.870 - 1.930 |
Rwork | 0.181 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p0z |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.870 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.080 | |
Number of reflections | 22900 | |
<I/σ(I)> | 12.9 | |
Completeness [%] | 96.7 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 293 | precipitant solution: 0.2 M Ammonium citrate tribasic, pH 7.0, 20 % (w/v) PEG 3,350 protein buffer: 25 mM HEPES pH 7.6, 75 mM Na2SO4 |