6G1B
Corynebacterium glutamicum OxyR, oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.307, 46.186, 116.692 |
| Unit cell angles | 90.00, 121.92, 90.00 |
Refinement procedure
| Resolution | 99.050 - 2.280 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.23020 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.471 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.070 | 46.070 | 2.360 |
| High resolution limit [Å] | 2.280 | 8.850 | 2.280 |
| Rmerge | 0.120 | 0.069 | 0.955 |
| Rmeas | 0.134 | 0.079 | 1.062 |
| Rpim | 0.059 | 0.036 | 0.459 |
| Total number of observations | 165386 | 2699 | 15489 |
| Number of reflections | 32352 | 579 | 3025 |
| <I/σ(I)> | 8.1 | 24.6 | 1.5 |
| Completeness [%] | 96.6 | 91.6 | 93.3 |
| Redundancy | 5.1 | 4.7 | 5.1 |
| CC(1/2) | 0.993 | 0.987 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 4000, sodium cacodylate, magnesium acetate, nicotinamide adenine dinucelotide hydrate |
