6G16

Structure of the human RBBP4:MTA1(464-546) complex showing loop exchange

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 1 21 1
Unit cell lengths	82.232, 150.352, 95.694
Unit cell angles	90.00, 94.99, 90.00

Refinement procedure

Resolution	95.330 - 2.800
R-factor	0.2264
Rwork	0.224
R-free	0.26328
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fxy
RMSD bond length	0.012
RMSD bond angle	1.442
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.400	2.880
High resolution limit [Å]	2.800	2.800
Rmerge	0.100	0.820
Number of reflections	51450
<I/σ(I)>	15.5	3.4
Completeness [%]	95.0	96.9
Redundancy	9.3	9.2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.21 M AMMONIUM CITRATE, 19% PEG 3350, 15 mM MALTOSE pH 7.5