6G15

Crystal structure of pppGpp bound RbgA from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	72.084, 78.467, 125.022
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.893 - 1.650
R-factor	0.2193
Rwork	0.218
R-free	0.24430
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.893	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	164656
<I/σ(I)>	24.7
Completeness [%]	100.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Potassium fluoride, 0.1 M MES pH 6.0, 20% PEG 3350