6G14

Crystal structure of ppGpp bound RbgA from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	71.781, 74.512, 125.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.933 - 1.800
R-factor	0.2195
Rwork	0.218
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g0z
RMSD bond length	0.009
RMSD bond angle	1.192
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.933	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	115942
<I/σ(I)>	7.88
Completeness [%]	99.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Potassium sulfate, 20% PEG 3350