6G10
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with the HMA domain of Pikp-1 from rice (Oryza sativa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.500, 66.261, 45.739 |
Unit cell angles | 90.00, 113.32, 90.00 |
Refinement procedure
Resolution | 42.000 - 1.350 |
R-factor | 0.1447 |
Rwork | 0.143 |
R-free | 0.18355 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a6w |
RMSD bond length | 0.018 |
RMSD bond angle | 1.919 |
Data reduction software | xia2 (0.5.328) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.061 | |
Number of reflections | 48646 | |
<I/σ(I)> | 13.4 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.12M Alcohols (0.2M 1,6-Hexanediol; 0.2M 1-Butanol; 0.2M 1,2-Propanediol; 0.2M 2-Propanol; 0.2M 1,4-Butanediol; 0.2M 1,3-Propanediol); 0.1M Buffer system 1 (1M Imidazole; MES monohydrate (acid)) pH 6.5; 50% v/v Precipitant mix 4 (25%v/v MPD; 25%v/v PEG 1000; 25%v/v PEG3350) |