6G0Z
Crystal structure of GDP bound RbgA from S. aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.932, 77.812, 124.667 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.649 - 2.150 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1puj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.212 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.650 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 73381 | |
<I/σ(I)> | 2.45 | |
Completeness [%] | 99.8 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Lithium sulfate, 0.1 M MES pH 6.0, 35% (v/v) MPD |