6G0Y
X-ray structure of M-21 protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.555, 116.524, 72.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.350 - 2.420 |
| R-factor | 0.22282 |
| Rwork | 0.220 |
| R-free | 0.28035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.729 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.350 | 2.480 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.150 | 0.950 |
| Rmeas | 0.160 | 1.000 |
| Rpim | 0.060 | 0.390 |
| Number of reflections | 32012 | 2309 |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 100.0 | 98.6 |
| Redundancy | 12.6 | 13 |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | The crystallisation plate was incluabed in 20 degree celcius for crystal growth. The reservoir had 500 micro litre of 20% PEG-MME 2000, 0.1M TRis (pH 8.5), 0.2M trimethylamine N-oxide. The 2microlitre protein-peptide sample was mixed with 1ul of reservoir solution and placed in the cover slip for hanging drop set up |
