6G0X
TAILSPIKE PROTEIN OF E. COLI BACTERIOPHAGE HK620 IN COMPLEX WITH PENTASACCHARIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.320, 74.320, 174.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.210 - 1.410 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vji |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.914 |
| Data reduction software | XSCALE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.070 | 0.728 |
| Number of reflections | 107055 | |
| <I/σ(I)> | 17 | 2 |
| Completeness [%] | 98.6 | 87.8 |
| Redundancy | 5.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M TRIS-HCL, 3.5 M SODIUMFORMIATE |
