6G09
Crystal Structure of a GH8 xylobiose complex from Teredinibacter turnerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.702, 79.035, 87.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.490 - 1.400 |
| R-factor | 0.15611 |
| Rwork | 0.155 |
| R-free | 0.17737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6g00 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.801 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.490 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.051 | 0.549 |
| Number of reflections | 84123 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1 M sodium acetate, pH 5, 0.2 M ammonium sulfate, 20 % PEG 6000 |
