6G02
Complex of neuraminidase from H1N1 influenza virus with tamiphosphor omega-azidohexyl ester
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 4 |
| Unit cell lengths | 114.860, 114.860, 63.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.710 - 1.840 |
| R-factor | 0.15445 |
| Rwork | 0.153 |
| R-free | 0.19671 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.660 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.720 | 1.960 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmeas | 0.138 | 0.782 |
| Number of reflections | 71773 | 11457 |
| <I/σ(I)> | 9.06 | 1.74 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 3.74 | 3.55 |
| CC(1/2) | 0.994 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM HEPES pH 7.0, 8% PEG 8000 |
