6G01
Complex of neuraminidase from H1N1 influenza virus with tamiphosphor monomethyl ester
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.583, 137.408, 118.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.890 - 1.610 |
| R-factor | 0.17826 |
| Rwork | 0.178 |
| R-free | 0.20485 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 1.710 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmeas | 0.095 | 0.645 |
| Number of reflections | 121339 | 19127 |
| <I/σ(I)> | 10.29 | 1.88 |
| Completeness [%] | 98.2 | 96.4 |
| Redundancy | 3.13 | 2.79 |
| CC(1/2) | 0.997 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M HEPES, pH 7.75, 5% PEG 8000 |
