6FZY
PPAR mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.190, 62.670, 165.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.650 - 3.100 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23480 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.144 | 3.211 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.080 | |
| Number of reflections | 12300 | |
| <I/σ(I)> | 16.78 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.5 M ammonium sulfate, 0.1 M PIPES pH7, 0.9 M disodium tartrate |
