6FZN
SMURFP-Y56R mutant in complex with biliverdin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.550, 57.960, 52.110 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21475 |
| Rwork | 0.212 |
| R-free | 0.26258 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.800 |
| Number of reflections | 8452 | |
| <I/σ(I)> | 10.83 | 1.99 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.999 | 0.811 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 2% v/v 1,4-dioxane, 0.1 M Tris pH 8.0, 15% w/v Polyethylene glycol 3,350 |
