6FZ8

Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F/A187F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07227
Spacegroup name	P 21 21 21
Unit cell lengths	49.720, 71.870, 114.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.115 - 2.200
R-factor	0.2241
Rwork	0.224
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x6u
RMSD bond length	0.027
RMSD bond angle	1.887
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.870	2.316
High resolution limit [Å]	2.200	2.200
Rmerge	0.104
Rmeas	0.118
Number of reflections	21175
<I/σ(I)>	10
Completeness [%]	98.5
Redundancy	4.5
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium formate, 20% PEG 3350