6FZ0
Crystal structure of the metY SAM V riboswitch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9196 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 87.828, 87.828, 62.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.113 - 2.499 |
R-factor | 0.2338 |
Rwork | 0.231 |
R-free | 0.26990 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.601 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.113 | 2.540 |
High resolution limit [Å] | 2.499 | 2.500 |
Rmerge | 0.083 | 0.976 |
Rmeas | 0.086 | |
Rpim | 0.023 | 0.276 |
Number of reflections | 15977 | 428 |
<I/σ(I)> | 16.8 | 2.1 |
Completeness [%] | 100.0 | 99.3 |
Redundancy | 13.8 | 13.2 |
CC(1/2) | 0.999 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 280 | 0.2MPotassium chloride 0.01MCalcium chloride dihydrate 0.05MBis-Tris7.0 40% v/vPEG 400 |